Photoelectrochemical identification of 5-methylcytosine modification in DNA: combination of photosensitization and enzymatic cleavage

Nucleic Acids Symp Ser (Oxf). 2009;(53):205-6. doi: 10.1093/nass/nrp103.

Abstract

We present a novel photoelectrochemical approach for discriminating between cytosine and 5-methylcytosine in DNA when used in combination with enzymatic digestion. A photosensitizer-linked DNA duplex bearing 5-methylcytosine or cytosine at a given restriction site of the strand was immobilized on a gold electrode and digested with enzyme, and the photocurrent response was measured. We observed high photocurrent density that was selective for the methylated duplex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Methylcytosine / analysis*
  • 5-Methylcytosine / chemistry
  • Cytosine / analysis
  • DNA / chemistry*
  • DNA Methylation
  • DNA Restriction Enzymes
  • Oligodeoxyribonucleotides / chemistry
  • Photochemical Processes
  • Photochemistry / methods

Substances

  • Oligodeoxyribonucleotides
  • 5-Methylcytosine
  • Cytosine
  • DNA
  • DNA Restriction Enzymes