Heat-shock protein 65 as a beta cell antigen of insulin-dependent diabetes

Lancet. 1990 Sep 8;336(8715):583-5. doi: 10.1016/0140-6736(90)93390-b.


The primary beta-cell antigen of insulin-dependent diabetes is thought to be a protein with a molecular weight of approximately 64 kD. Hyperthermic incubation and cytokines such as interleukin 1 beta, gamma interferon, and tumour necrosis factor induce synthesis of 64 kD protein by insulinoma cells. By western blot techniques, cross-reactivity was found between this 64 kD protein and monoclonal antibodies directed against Mycobacterium tuberculosis heat-shock protein 65, but not with antibodies directed against a similar epitope of M leprae heat-shock protein 65. Binding of M tuberculosis heat-shock protein 65 antibodies to interleukin-1 beta-treated cells was inhibited by prior addition of serum from insulin-dependent diabetic patients which contained antibodies to 64 kD beta-cell antigen. It is suggested that heat-shock protein 65 may be the 64 kD beta-cell antigen and that autoreactivity to an epitope of heat-shock protein 65 may confer susceptibility to insulin-dependent diabetes mellitus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Neoplasm / analysis*
  • Autoradiography
  • Cross Reactions
  • Diabetes Mellitus, Type 1 / immunology*
  • Diabetes Mellitus, Type 1 / metabolism
  • Heat-Shock Proteins / analysis*
  • Heat-Shock Proteins / immunology*
  • Heat-Shock Proteins / metabolism
  • Humans
  • Insulinoma / drug therapy
  • Insulinoma / immunology
  • Interleukin-1 / therapeutic use
  • Pancreatic Neoplasms / drug therapy
  • Pancreatic Neoplasms / immunology
  • Rats
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Heat-Shock Proteins
  • Interleukin-1