An investigation of serum concentration of apoM as a potential MODY3 marker using a novel ELISA

J Intern Med. 2010 Mar;267(3):316-21. doi: 10.1111/j.1365-2796.2009.02145.x. Epub 2009 Jun 22.


Objective: To investigate the fitness of serum apolipoprotein M (apoM) concentration as a marker for maturity-onset diabetes of the young 3 (MODY3).

Study design and subjects: This study consisted of two parts. A family study included 71 carriers of the P291fsinsC mutation in hepatocyte nuclear factor-1alpha (HNF-1alpha) from the Finnish Botnia study, 53 of whom were diabetic, and 75 matched family controls. A second, case-control study included 24 MODY3 patients, 17 healthy MODY3 mutation carriers, 11 MODY1 patients, 18 type 2 diabetes patients and 19 healthy control individuals. Subjects in the case-control study were recruited from the Botnia study or the Clinic of Endocrinology, Malmö University Hospital. Serum apoM levels were measured using a novel ELISA based on two monoclonal apoM antibodies.

Results: In the family study, mean serum apoM was 10% lower in female carriers of the P291fsinsC mutation compared to the family controls (P = 0.0058), a difference which remained significant after adjustment for diabetes status. There was no observed difference between groups for men. In the case-control study, no significant difference in apoM concentration was observed between MODY3 and type 2 diabetes patients, neither before nor after adjustment for total cholesterol.

Conclusions: Female carriers of the P291fsinsC mutation in HNF-1alpha displayed slightly lower apoM serum levels. This difference is too small for apoM to be reliably employed as a biomarker for HNF-1alpha mutation status.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apolipoproteins / blood*
  • Apolipoproteins M
  • Biomarkers / blood
  • Case-Control Studies
  • Diabetes Mellitus, Type 2 / blood*
  • Diabetes Mellitus, Type 2 / genetics
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Genetic Predisposition to Disease
  • Hepatocyte Nuclear Factor 1-alpha / genetics
  • Heterozygote
  • Humans
  • Lipocalins
  • Male
  • Middle Aged
  • Mutation
  • Predictive Value of Tests
  • Sex Factors


  • APOM protein, human
  • Apolipoproteins
  • Apolipoproteins M
  • Biomarkers
  • Hepatocyte Nuclear Factor 1-alpha
  • Lipocalins