Abstract
This study examined the analgesic and anti-inflammatory actions of cis-mulberroside A isolated from Ramulus mori in several models of inflammatory pain in mice. Cis-mulberroside A (25 and 50mg/kg) given by p.o. route 30 min before challenge produced a dose-dependent inhibition of the acetic acid-induced pain and Evans blue leakage in mice. In addition, this compound exhibited significant systemic anti-inflammatory activity in carrageenan-induced mouse paw edema in a concentration-related manner (33.1-68.5% inhibition), and similar results were achieved in formalin test. Suppressive effects of cis-mulberroside A on the production of NO and expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated macrophages were also assessed. Collectively, cis-mulberroside A showed high analgesic and anti-inflammatory activities. The above results will be the supporting evidence for the potential anti-rheumatoid activity of R.mori in Chinese traditional medicine.
Copyright (c) 2009 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetic Acid
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Analgesics / isolation & purification
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Analgesics / pharmacology
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Analgesics / therapeutic use*
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Animals
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology
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Anti-Inflammatory Agents / therapeutic use*
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Carrageenan
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Cell Line
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Disaccharides / isolation & purification
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Disaccharides / pharmacology
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Disaccharides / therapeutic use*
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Dose-Response Relationship, Drug
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Edema / chemically induced
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Edema / drug therapy*
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Evans Blue
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Formaldehyde
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Inflammation / drug therapy
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Lipopolysaccharides
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Macrophages / drug effects*
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Male
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Mice
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Mice, Inbred ICR
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Moraceae / chemistry*
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Nitric Oxide / biosynthesis
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Nitric Oxide Synthase Type II / metabolism
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Pain / drug therapy
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Phytotherapy
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Plant Extracts / chemistry
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use*
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Plant Stems
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Stilbenes / isolation & purification
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Stilbenes / pharmacology
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Stilbenes / therapeutic use*
Substances
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Analgesics
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Anti-Inflammatory Agents
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Disaccharides
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Lipopolysaccharides
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Plant Extracts
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Stilbenes
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mulberroside A
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Formaldehyde
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Nitric Oxide
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Evans Blue
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Carrageenan
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Nitric Oxide Synthase Type II
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Acetic Acid