Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization

Comb Chem High Throughput Screen. 2009 Sep;12(8):791-800. doi: 10.2174/138620709789104915.

Abstract

The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.

Publication types

  • Review

MeSH terms

  • Antibodies / chemistry
  • Antigen-Antibody Reactions
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Drug Discovery / methods
  • Interferometry / methods*
  • Kinetics
  • Ligands
  • Proteins / chemistry
  • Staining and Labeling
  • Time Factors

Substances

  • Antibodies
  • Ligands
  • Proteins