Abstract
We have previously shown that T cells bearing V beta 5+ T-cell receptors (TCRs) are frequent in B10 (H-2b) and B10.Q (H-2q) mouse strains but are rare in the congenic strain B10.BR (H-2k). Furthermore, we have found that V beta 5 bearing T cells appear to be excluded from the B10 alloresponse to I-Abm12 despite the participation of most other V beta bearing cells. To further study MHC effects on V beta 5 expression, we have generated two V beta 5 specific monoclonal antibodies and show here that V beta 5 expressing T cells are clonally deleted from strains expressing a class II, I-E molecule. Furthermore, I-E- strains generate few CD4+ V beta 5+ T cells despite significant numbers of V beta 5+ T cells in the CD8+ subset. Thus, V beta 5 bearing T cells are positively selected by class I MHC molecules, clonally deleted by class II I-E molecules, and poorly selected by class II I-A molecules.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / biosynthesis
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Antibodies, Monoclonal / immunology
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Base Sequence
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CD4-Positive T-Lymphocytes / immunology
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DNA
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Flow Cytometry
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H-2 Antigens / immunology
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Histocompatibility Antigens Class I / genetics
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Histocompatibility Antigens Class I / physiology*
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Histocompatibility Antigens Class II / genetics
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Histocompatibility Antigens Class II / immunology
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Histocompatibility Antigens Class II / physiology*
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Hybrid Cells
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Mice
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Mice, Inbred Strains
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Molecular Sequence Data
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / immunology*
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Receptors, Antigen, T-Cell, alpha-beta
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T-Lymphocytes / immunology*
Substances
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Antibodies, Monoclonal
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H-2 Antigens
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Histocompatibility Antigens Class I
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Histocompatibility Antigens Class II
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I-E-antigen
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Receptors, Antigen, T-Cell
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Receptors, Antigen, T-Cell, alpha-beta
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DNA