Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases

Yajun Yan; Chia-Chi Lee; James C Liao

doi:10.1039/b913501d

Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases

Org Biomol Chem. 2009 Oct 7;7(19):3914-7. doi: 10.1039/b913501d. Epub 2009 Aug 3.

Authors

Yajun Yan¹, Chia-Chi Lee, James C Liao

Affiliation

¹ Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531, Boelter Hall, 420 Westwood Plaza, Los Angeles, CA 90095, USA.

PMID: 19763290
DOI: 10.1039/b913501d

Abstract

We characterized the activity and stereospecificity of four secondary alcohol dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in E. coli to produce enantiomerically pure (R,R)-2,3-butanediol (2,3-BDO) from glucose with a titer of 6.1 g/L (enantio purity >99%), and yield of 0.31 g product/g glucose (62% of theoretical maximum).

MeSH terms

Alcohol Oxidoreductases / metabolism*
Butylene Glycols / chemistry*
Butylene Glycols / metabolism*
Escherichia coli / enzymology*
Kinetics
Stereoisomerism
Substrate Specificity

Substances

Butylene Glycols
Alcohol Oxidoreductases
isopropanol dehydrogenase (NADP)