Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity

EMBO J. 1990 Nov;9(11):3631-9.

Abstract

The cDNA for TTF-1, a thyroid nuclear factor that binds to the promoter of thyroid specific genes, has been cloned. The protein encoded by the cDNA shows binding properties indistinguishable from those of TTF-1 present in nuclear extracts of differentiated rat thyroid cells. The DNA binding domain of TTF-1 is a novel mammalian homeodomain that shows considerable sequence homology to the Drosophila NK-2 homeodomain. TTF-1 mRNA and corresponding binding activity are detected in thyroid and lung. The chromosomal localization of the TTF-1 gene has been determined in humans and mice and corresponds to chromosomes 14 and 12, respectively, demonstrating that the TTF-1 gene is not located within previously described clusters of homeobox-containing genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Chromosome Mapping
  • Chromosomes, Human, Pair 14
  • Cloning, Molecular
  • DNA / genetics
  • DNA-Binding Proteins / genetics*
  • Gene Expression
  • Genes, Homeobox
  • Humans
  • Lung / physiology
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • RNA, Messenger / genetics
  • Rats
  • Regulatory Sequences, Nucleic Acid
  • Thyroid Gland / physiology*
  • Thyroid Nuclear Factor 1
  • Tissue Distribution
  • Transcription Factors*

Substances

  • DNA-Binding Proteins
  • NKX2-1 protein, human
  • Nkx2-1 protein, mouse
  • Nkx2-1 protein, rat
  • Nuclear Proteins
  • RNA, Messenger
  • Thyroid Nuclear Factor 1
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/X53858