Interleukin-1 attenuates normal tension glaucoma-like retinal degeneration in EAAC1-deficient mice

Neurosci Lett. 2009 Nov 13;465(2):160-4. doi: 10.1016/j.neulet.2009.09.029. Epub 2009 Sep 17.


Glaucoma, one of the leading causes of irreversible blindness, is characterized by progressive degeneration of retinal ganglion cells (RGCs) and optic nerves. Although glaucoma is often associated with elevated intraocular pressure, recent studies have shown a relatively high prevalence of normal tension glaucoma (NTG) in glaucoma patient populations. In the mammalian retina, glutamate/aspartate transporter (GLAST) is localized to Müller glial cells, whereas excitatory amino acid carrier 1 (EAAC1) is expressed in neural cells, including RGCs. Since the loss of GLAST or EAAC1 leads to retinal degeneration similar to that seen in NTG, we examined the effects of interleukin-1 (IL-1) on RGC death in GLAST- and EAAC1-deficient mice. IL-1 promoted increased glutamate uptake in Müller cells by suppressing intracellular Na(+) accumulation, which is necessary to counteract Na(+)-glutamate cotransport. The observed trends for the glutamate uptake increase in the wild-type (WT), GLAST- and EAAC1-deficient mice were similar; however, the baseline glutamate uptake and intracellular Na(+) concentration in the GLAST-deficient mice were significantly lower than those in the wild-type mice. Consistently, pretreatment with IL-1 exhibited no beneficial effects on glutamate-induced RGC degeneration in the GLAST-deficient mice. In contrast, IL-1 significantly increased glutamate uptake by Müller cells and the number of surviving RGCs in the wild-type and EAAC1-deficient mice. Our findings suggest that the use of IL-1 for enhancing the function of glutamate transporters may be useful for neuroprotection in retinal degenerative disorders including NTG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Death / physiology
  • Cell Survival / physiology
  • Excitatory Amino Acid Transporter 1 / deficiency*
  • Excitatory Amino Acid Transporter 1 / genetics
  • Excitatory Amino Acid Transporter 1 / metabolism
  • Excitatory Amino Acid Transporter 3 / deficiency*
  • Excitatory Amino Acid Transporter 3 / genetics
  • Excitatory Amino Acid Transporter 3 / metabolism
  • Glaucoma / physiopathology
  • Glutamic Acid / metabolism
  • Interleukin-1 / metabolism*
  • Intracellular Space / metabolism
  • Intraocular Pressure
  • Mice
  • Mice, Knockout
  • Neuroglia / physiology
  • Retina / physiopathology*
  • Retinal Degeneration / physiopathology*
  • Retinal Ganglion Cells / physiology*
  • Sodium / metabolism


  • Excitatory Amino Acid Transporter 1
  • Excitatory Amino Acid Transporter 3
  • Interleukin-1
  • Slc1a1 protein, mouse
  • Slc1a3 protein, mouse
  • Glutamic Acid
  • Sodium