Abstract
The gene encoding a novel modular xylanase from Cellulosimicrobium sp. strain HY-13 was identified and expressed in Escherichia coli, and its truncated gene product was characterized. The enzyme consisted of three distinct functional domains, an N-terminal catalytic GH10 domain, a fibronectin type 3 domain, and C-terminal carbohydrate-binding module 2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinomycetales / enzymology*
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Actinomycetales / genetics
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Amino Acid Sequence
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Animals
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Endo-1,4-beta Xylanases / chemistry
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Endo-1,4-beta Xylanases / genetics
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Endo-1,4-beta Xylanases / metabolism
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Fibronectins / chemistry
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Intestines / microbiology
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Molecular Sequence Data
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Oligochaeta / microbiology*
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Protein Binding
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Protein Structure, Tertiary
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Sequence Alignment
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Substrate Specificity / genetics
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Xylosidases / chemistry
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Xylosidases / genetics
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Xylosidases / metabolism*
Substances
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Fibronectins
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Xylosidases
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Endo-1,4-beta Xylanases