Genetics and biology of human ovarian teratomas. II. Molecular analysis of origin of nondisjunction and gene-centromere mapping of chromosome I markers

Am J Hum Genet. 1990 Oct;47(4):644-55.

Abstract

Chromosomal heteromorphisms and DNA polymorphisms have been utilized to identify the mechanisms that lead to formation of human ovarian teratomas and to construct a gene-centromere map of chromosome 1 by using those teratomas that arise by meiotic nondisjunction. Of 61 genetically informative ovarian teratomas, 21.3% arose by nondisjunction at meiosis I, and 39.3% arose by meiosis II nondisjunction. Eight polymorphic marker loci on chromosome 1p and one marker on 1q were used to estimate a gene-centromere map. The results show clear linkage of the most proximal 1p marker (NRAS) and the most proximal 1q marker (D1S61) to the centromere at a distance of 14 cM and 20 cM, respectively. Estimated gene-centromere distances suggest that, while recombination occurs normally in ovarian teratomas arising by meiosis II errors, ovarian teratomas arising by meiosis I nondisjunction have altered patterns of recombination. Furthermore, the estimated map demonstrates clear evidence of chiasma interference. Our results suggest that ovarian teratomas can provide a rapid method for mapping genes relative to the centromere.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Centromere*
  • Child
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1*
  • Chromosomes, Human, Pair 13
  • Chromosomes, Human, Pair 21
  • DNA Probes
  • DNA, Neoplasm / genetics
  • Female
  • Gene Frequency
  • Genetic Markers
  • Humans
  • Middle Aged
  • Nondisjunction, Genetic*
  • Ovarian Neoplasms / etiology
  • Ovarian Neoplasms / genetics*
  • Polymorphism, Restriction Fragment Length
  • Teratoma / etiology
  • Teratoma / genetics*

Substances

  • DNA Probes
  • DNA, Neoplasm
  • Genetic Markers