Cloning and structure of cDNA encoding alpha-latrotoxin from black widow spider venom

FEBS Lett. 1990 Sep 17;270(1-2):127-31. doi: 10.1016/0014-5793(90)81250-r.


cDNA encoding the putative alpha-latrotoxin precursor was isolated from spider venom glands cDNA library and sequenced. The cDNA contained the 4203 base-pair open reading frame corresponding to the 156,855-Da protein composed of 1401 amino acids. Computer analysis of the deduced primary structure revealed the presence of various internal imperfect repeats mainly in its central and C-terminal regions.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Gene Library
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Poly A / genetics
  • RNA, Messenger / genetics
  • Repetitive Sequences, Nucleic Acid
  • Spider Venoms / chemistry
  • Spider Venoms / genetics*


  • Peptide Fragments
  • RNA, Messenger
  • Spider Venoms
  • Poly A
  • alpha-latrotoxin