Restriction fragment length polymorphism DNA analysis by the FBI Laboratory protocol using a simple, convenient hardware system

M E Lewis; R E Kouri; D Latorra; K M Berka; H C Lee; R E Gaensslen

Restriction fragment length polymorphism DNA analysis by the FBI Laboratory protocol using a simple, convenient hardware system

J Forensic Sci. 1990 Sep;35(5):1186-90.

Authors

M E Lewis¹, R E Kouri, D Latorra, K M Berka, H C Lee, R E Gaensslen

Affiliation

¹ Laboratory of Molecular Biology, BIOS Corp., New Haven, CT.

PMID: 1977839

Abstract

Restriction fragment length polymorphism analysis of human deoxyribonucleic acid (DNA) using two probes, pYNH24 and CMM101, was performed on the BIOS Timeframe system following the Federal Bureau of Investigation (FBI) Laboratory protocol and some variations of it. Comparable results were obtained by the different methods used.

MeSH terms

Autoradiography
Blotting, Southern
Cells, Cultured
Computers*
DNA / analysis*
DNA Probes
Electrophoresis, Agar Gel
Forensic Medicine / methods*
Humans
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length*
Restriction Mapping

Substances

DNA Probes
DNA