Bid cleavage, cytochrome c release and caspase activation in canine coronavirus-induced apoptosis

Vet Microbiol. 2010 Feb 24;141(1-2):36-45. doi: 10.1016/j.vetmic.2009.09.001. Epub 2009 Sep 11.


A previous study demonstrated that infection of a canine fibrosarcoma cell line (A-72 cells) by canine coronavirus (CCoV) resulted in apoptosis (Ruggieri et al., 2007). In this study, we investigated the cell death processes during infection and the underlying mechanisms. We found that CCoV-II triggers apoptosis in A-72 cells by activating initiator (caspase-8 and -9) and executioner (caspase-3 and -6) caspases. The proteolytic cleavage of poly(ADP-ribose) polymerases (PARPs) confirmed the activation of executioner caspases. Furthermore, CCoV-II infection resulted in truncated bid (tbid) translocation from the cytosolic to the mitochondrial fraction, the cytochrome c release from mitochondria, and alterations in the pro- and anti-apoptotic proteins of bcl-2 family. Our data indicated that, in this experimental model, both intrinsic and extrinsic pathways are involved. In addition, we demonstrated that the inhibition of apoptosis by caspase inhibitors did not affect CCoV replication, suggesting that apoptosis does not play a role in facilitating viral release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Apoptosis / genetics
  • Apoptosis / physiology*
  • Blotting, Western
  • Caspase 3 / metabolism
  • Caspase 6 / metabolism
  • Caspase 8 / metabolism
  • Caspase 9 / metabolism
  • Caspases / metabolism*
  • Cell Line, Tumor
  • Cell Survival / genetics
  • Cell Survival / physiology
  • Coronavirus, Canine / physiology*
  • Cysteine Proteinase Inhibitors / pharmacology
  • Cytochromes c / metabolism*
  • Dogs
  • Flow Cytometry


  • Cysteine Proteinase Inhibitors
  • Cytochromes c
  • Caspase 3
  • Caspase 6
  • Caspase 8
  • Caspase 9
  • Caspases