Down-regulation of dicer expression in ovarian cancer tissues

Clin Biochem. 2010 Feb;43(3):324-7. doi: 10.1016/j.clinbiochem.2009.09.014. Epub 2009 Sep 25.

Abstract

Objectives: Although numerous recent studies have focused on analyses of microRNA expression profiles in cancer cells, the expression patterns of the enzymes responsible for the generation of miRNAs remains largely unexplored. The purpose of this study was to investigate whether Dicer mRNA expression is altered during progression of ovarian cancer.

Design and methods: Total RNA was extracted from ovarian tissue specimens (normal, benign and malignant tumors). The expression of Dicer was analyzed by semi-quantitative RT-PCR.

Results: We analyzed a total of 34 ovarian tissue samples and found that Dicer mRNA expression is down-regulated in the majority of ovarian tumors when compared to normal tissues.

Conclusions: Our results suggest that the levels of Dicer mRNA should be evaluated as a potential new candidate biomarker for ovarian cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism
  • DEAD-box RNA Helicases* / genetics
  • DEAD-box RNA Helicases* / metabolism
  • Disease Progression
  • Down-Regulation*
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • Middle Aged
  • Ovarian Neoplasms / genetics*
  • Ovarian Neoplasms / metabolism*
  • Ovarian Neoplasms / pathology
  • Ovary / metabolism
  • Ovary / pathology
  • Ribonuclease III* / genetics
  • Ribonuclease III* / metabolism

Substances

  • Biomarkers, Tumor
  • MicroRNAs
  • DICER1 protein, human
  • Ribonuclease III
  • DEAD-box RNA Helicases