Mammalian polymerase zeta is essential for post-replication repair of UV-induced DNA lesions

DNA Repair (Amst). 2009 Dec 3;8(12):1444-51. doi: 10.1016/j.dnarep.2009.09.006. Epub 2009 Sep 27.


DNA polymerase zeta is believed to be an essential constituent of DNA damage tolerance, comprising several pathways that allow the replication of DNA templates containing unrepaired damage. We wanted to better define the role of polymerase zeta in DNA damage tolerance in mammalian cells. To this aim we have investigated replication of ultraviolet light-damaged DNA templates in mouse embryonic fibroblasts deficient for Rev3, the catalytic subunit of polymerase zeta. We found that Rev3 is important for a post-replication repair pathway of helix-distorting [6-4]pyrimidine-pyrimidone photoproducts and, to a lesser extent, of cyclobutane pyrimidine dimers. Unlike its partner Rev1, Rev3 appears not to be involved in an immediate translesion synthesis pathway at a stalled replication fork. The deficiency of Rev3(-/-) MEFs in post-replication repair of different photoproducts contributes to the extreme sensitivity of these cells to UV light.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • DNA / metabolism*
  • DNA Repair*
  • DNA Replication
  • DNA-Directed DNA Polymerase / deficiency
  • DNA-Directed DNA Polymerase / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Ultraviolet Rays*


  • DNA
  • DNA polymerase zeta
  • Rev3 protein, mouse
  • DNA-Directed DNA Polymerase