Arthrobacter antarcticus sp. nov., isolated from an Antarctic marine sediment

Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2263-2266. doi: 10.1099/ijs.0.012989-0. Epub 2009 Sep 25.

Abstract

A bacterial strain, SPC26(T), was isolated from a sediment sample of the Southern Ocean off Antarctica. The strain was Gram-staining- and catalase-positive and contained lysine and alanine in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C₁₅:₀ (54.92 %), iso-C₁₅:₀ (11.47 %), anteiso-C₁₇:₀ (6.48 %) and anteiso-C₁₅:₁ (6.38 %) and the major menaquinones were MK-8, MK-9 and MK-10. The major polar lipids were phosphatidylethanolamine and diphosphatidylglycerol. The G+C content was 68 ± 0.5 mol%. Based on 16S rRNA gene sequence similarities, the nearest phylogenetic neighbours of strain SPC26(T) were identified as Arthrobacter gangotriensis Lz1y(T) (98.8 %), A. sulfureus DSM 20167(T) (98.6 %), A. psychrophenolicus DSM 15454(T) (97.9 %) and A. kerguelensis KGN15(T) (97.5). With these strains, strain SPC26(T) exhibited DNA-DNA relatedness values of 36, 21, 12 and 10 %, respectively. Therefore, on the basis of 16S rRNA gene sequence comparisons, phylogenetic analysis, phenotypic characteristics and DNA-DNA relatedness, it is proposed that strain SPC26(T) represents a novel species of Arthrobacter, for which the name Arthrobacter antarcticus sp. nov. is proposed, with strain SPC26(T) (=LMG 24542(T) =NCCB 100228(T)) as the type strain.

MeSH terms

  • Alanine / analysis
  • Antarctic Regions
  • Arthrobacter / chemistry
  • Arthrobacter / classification*
  • Arthrobacter / genetics
  • Arthrobacter / isolation & purification*
  • Bacterial Typing Techniques
  • Base Composition
  • Catalase / metabolism
  • Cell Wall / chemistry
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Geologic Sediments / microbiology*
  • Lysine / analysis
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Peptidoglycan / analysis
  • Phospholipids / analysis
  • Phylogeny
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Peptidoglycan
  • Phospholipids
  • Quinones
  • RNA, Ribosomal, 16S
  • Catalase
  • Lysine
  • Alanine

Associated data

  • GENBANK/AM931709