Abstract
Leukemias/lymphomas with IGH-involving del(14q)(1) commonly lose the DLK1-GTL2 imprinted domain that comprises several paternally and maternally expressed genes, including a cluster of microRNAs. Given that deletion of this region could lead to inactivation of a monoallelically expressed tumor suppressor gene, our study aimed at determination of the parental origin of del(14q/IGH). The designed allele-specific methylation study of the DLK1/GTL2 intergenic differentially methylated region allowed us to determine the parental origin of del(14q/IGH) in 9/20 analyzed cases. In six cases del(14q/IGH) was of the paternal origin and in three cases of the maternal origin. These findings argue against the concept that a TSG/anti-oncomir located in the imprinted region is systematically inactivated by a targeted deletion of its functional allele.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Base Sequence
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Calcium-Binding Proteins
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Chromosomes, Human, Pair 14*
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Female
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Genes, Immunoglobulin Heavy Chain*
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Humans
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Intercellular Signaling Peptides and Proteins / analysis*
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism
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Leukemia, B-Cell / genetics
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Leukemia, B-Cell / immunology
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Leukemia, B-Cell / metabolism*
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Lymphoma, B-Cell / chemistry*
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Lymphoma, B-Cell / genetics
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Lymphoma, B-Cell / immunology
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Lymphoma, B-Cell / metabolism
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Male
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Membrane Proteins / analysis*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Methylation
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Parents
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Proteins / analysis*
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Proteins / genetics
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Proteins / metabolism
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RNA, Long Noncoding
Substances
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Calcium-Binding Proteins
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DLK1 protein, human
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Intercellular Signaling Peptides and Proteins
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MEG3 non-coding RNA, human
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Membrane Proteins
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Proteins
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RNA, Long Noncoding