Amplification and expression of the c-erb B-2/neu proto-oncogene in human bladder cancer

Mol Carcinog. 1990;3(5):254-7. doi: 10.1002/mc.2940030503.

Abstract

The c-erb B-2/neu gene encodes a cell-surface glycoprotein with extensive homology to, but distinct from, the epidermal growth-factor receptor. In this study, we compared the c-erb B-2/neu gene amplification and expression of tissue specimens obtained from the bladders of normal controls and patients with high-grade transitional cell bladder carcinoma. Southern blot analysis of DNAs from 24 patients and 5 controls showed 2 cases of c-erb B-2/neu gene amplification in patients and none in controls. Western blot analysis demonstrated that c-erb B-2/neu was expressed in 67.6% (23/34) of patient specimens but in none of the controls (0/5). This finding agreed with the result of immunohistochemical staining, which showed that tissue from 74.3% (26/35) of the patients and none of the controls (0/7) showed positive immunofluorescence staining. This is the first report suggesting that c-erb B-2/neu gene amplification may be associated with human bladder carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Southern
  • Blotting, Western
  • Carcinoma, Transitional Cell / genetics*
  • Carcinoma, Transitional Cell / immunology
  • DNA, Neoplasm / genetics
  • Fluorescent Antibody Technique
  • Gene Amplification*
  • Humans
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / immunology
  • Proto-Oncogene Proteins / genetics*
  • Receptor, ErbB-2
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / immunology

Substances

  • DNA, Neoplasm
  • Neoplasm Proteins
  • Proto-Oncogene Proteins
  • Receptor, ErbB-2