Analysis of protein conformation and dynamics by hydrogen/deuterium exchange MS

Anal Chem. 2009 Oct 1;81(19):7870-5. doi: 10.1021/ac901154s.


Understanding as much as possible about proteins in the shortest amount of time has long been a goal of hydrogen exchange (HX) MS. Recent technological advances have led to improvements in the technique, but has this goal yet been achieved? (To listen to a podcast about this Feature, please go to the Analytical Chemistry Web site at

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antibodies, Monoclonal / chemistry*
  • Antibodies, Monoclonal / immunology
  • Deuterium / chemistry*
  • Deuterium Exchange Measurement / methods*
  • Hydrogen / chemistry*
  • Isotope Labeling
  • Mass Spectrometry*
  • Protein Conformation
  • Recombinant Proteins / chemistry


  • Antibodies, Monoclonal
  • Recombinant Proteins
  • Hydrogen
  • Deuterium