Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera

Anal Biochem. 2010 Feb 1;397(1):129-31. doi: 10.1016/j.ab.2009.09.041. Epub 2009 Sep 27.


We found that certain mid-range consumer-level digital single-lens reflex (SLR) cameras using full-frame complementary metal oxide semiconductor (CMOS) sensors outperform X-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to X-ray film, yet they provide a 3-fold increase in linear dynamic range and substantial cost savings over time, are more convenient to use, and eliminate the chemical waste associated with processing film.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Technical Report

MeSH terms

  • Blotting, Western / methods*
  • Luminescent Measurements / instrumentation*
  • Luminescent Measurements / methods
  • Metals / chemistry
  • Oxides / chemistry
  • Semiconductors
  • X-Ray Film


  • Metals
  • Oxides