Molecular evolution of the Escherichia coli chromosome. III. Clonal frames

Genetics. 1990 Nov;126(3):505-17. doi: 10.1093/genetics/126.3.505.


PCR fragments, 1500-bp, from 15 previously sequenced regions in the Escherichia coli chromosome have been compared by restriction analysis in a large set of wild (ECOR) strains. Prior published observations of segmental clonality are confirmed: each of several sequence types is shared by a number of strains. The rate of recombinational replacement and the average size of the replacements are estimated in a set of closely related strains in which a clonal frame is dotted with occasional stretches of DNA belonging to other clones. A clonal hierarchy is described. Some new comparative sequencing data are presented.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Biological Evolution*
  • Chromosomes, Bacterial*
  • Cloning, Molecular
  • Escherichia coli / genetics*
  • Operon
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Sequence Homology, Nucleic Acid