Genetic analysis of meiotic recombination in Schizosaccharomyces pombe

Methods Mol Biol. 2009;557:65-76. doi: 10.1007/978-1-59745-527-5_6.

Abstract

The fission yeast Schizosaccharomyces pombe is well-suited for studying meiotic recombination. Methods are described here for culturing S. pombe and for genetic assays ofintragenic recombination (gene conversion), intergenic recombination (crossing-over), and spore viability. Both random spore and tetrad analyses are described.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Cell Culture Techniques
  • Genetic Techniques*
  • Meiosis / genetics*
  • Models, Biological
  • Recombination, Genetic / genetics*
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / growth & development
  • Schizosaccharomyces / physiology
  • Spores, Fungal / genetics
  • Spores, Fungal / growth & development