Two dicarbonyl compounds, 3-deoxyglucosone and methylglyoxal, differentially modulate dermal fibroblasts

Matrix Biol. 2010 Mar;29(2):127-34. doi: 10.1016/j.matbio.2009.09.007. Epub 2009 Oct 1.


Advanced glycation endproducts accumulate on long-lived proteins such as collagens as a function of diet and age and mediate the cross-linking of those proteins causing changes in collagen pathophysiology resulting in the disruption of normal collagen matrix remodeling. Two commonly studied advanced glycation endproduct precursors 3-deoxyglucosone and methylglyoxal were investigated for their role in the modification of collagen and on extracellular matrix expression. Fibroblasts cultured on methylglyoxal cross-linked matrices increased the expression of collagen, active TGF-beta1, beta1-integrin, and decreased Smad7; whereas 3-deoxyglucosone decreased collagen, active TGF-beta1, beta1-integrin but increased Smad7. Purified collagen modified by 3-deoxyglucosone or methylglyoxal had different molecular weights; methylglyoxal increased the apparent molecular weight by approximately 20 kDa, whereas 3-deoxyglucosone did not. The differences in collagen expression by 3-deoxyglucosone and methylglyoxal raise the provocative idea that a genetic or environmental background leading to the predominance of one of these advanced glycation endproduct precursors may precipitate a fibrotic or chronic wound in susceptible individuals, particularly in the diabetic.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Line
  • Collagen / chemistry
  • Collagen / metabolism
  • Deoxyglucose / analogs & derivatives*
  • Deoxyglucose / metabolism
  • Extracellular Matrix / chemistry
  • Extracellular Matrix / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / physiology
  • Glycation End Products, Advanced / metabolism
  • Humans
  • Integrin beta1 / metabolism
  • Protein Isoforms / chemistry
  • Protein Isoforms / metabolism
  • Pyruvaldehyde / metabolism*
  • Skin* / cytology
  • Skin* / metabolism
  • Transforming Growth Factor beta1 / metabolism


  • Glycation End Products, Advanced
  • Integrin beta1
  • Protein Isoforms
  • Transforming Growth Factor beta1
  • Pyruvaldehyde
  • Collagen
  • Deoxyglucose
  • 3-deoxyglucosone