Two-dimensional pulsed electron spin resonance characterization of 15N-labeled archaeal Rieske-type ferredoxin

FEBS Lett. 2009 Nov 3;583(21):3467-72. doi: 10.1016/j.febslet.2009.09.050. Epub 2009 Oct 3.


Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly (15)N-labeled archaeal Rieske-type [2Fe-2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high-potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non-coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Archaeal Proteins / chemistry*
  • Electron Spin Resonance Spectroscopy
  • Electron Transport Complex III / chemistry*
  • Ferredoxins / chemistry*
  • Isotope Labeling
  • Nitrogen Isotopes
  • Sulfolobus solfataricus


  • Archaeal Proteins
  • Ferredoxins
  • Nitrogen Isotopes
  • Rieske iron-sulfur protein
  • Electron Transport Complex III