Glucans from the Caripia montagnei mushroom present anti-inflammatory activity

Int Immunopharmacol. 2010 Jan;10(1):34-42. doi: 10.1016/j.intimp.2009.09.015. Epub 2009 Oct 3.

Abstract

Caripia montagnei is a basidiomycete species which contains polysaccharides with immunomodulatory properties. An extract of this mushroom underwent removal of the fat content by organic solvent and subsequently proteolysis. The aqueous phase obtained after proteolysis was precipitated with methanol yielding a fraction containing carbohydrates (98.7+/-3.3%) and protein (1.3+/-0.25%). Chemical analysis, infrared spectroscopy and nuclear magnetic resonance (NMR) showed that the carbohydrate fraction contained (63.3+/-4.1) of beta-glucans and proteins (2.2+/-0.3%). These glucans (50mg/kg of body weight) significantly reduced the inflammatory infiltrate produced by thioglycolate-induced peritonitis by 75.5+/-5.2%, when compared to Wy-14643 (60.3+/-6.1%), PFOA (37.8+/-2.8%) and clofibrate (52.2+/-3.2%), p<0.001, which are of the peroxisome proliferator-activated receptor (PPAR-alpha). L-NAME, a nitric oxide synthase inhibitor, reduced the plantar edema in Wistar rats by 91.4+/-1.3% (p<0.001). A significant reduction in nitric oxide (NO) levels was observed in the exudates when the glucans was used in comparison to carrageenan. The C. montagnei glucans did not present signs of inducing cytotoxicity. A decrease in IL-1ra, IL-10 and IFN-gamma in the peritonitis model was observed. Thus, the results suggest that glucans from the C. montagnei mushroom is an effective immunomodulator and may have potential for anti-inflammatory properties.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage*
  • Anti-Inflammatory Agents / adverse effects
  • Anti-Inflammatory Agents / chemistry
  • Ascitic Fluid / chemistry
  • Ascitic Fluid / immunology
  • Ascitic Fluid / pathology
  • Basidiomycota / immunology*
  • Caprylates / administration & dosage
  • Caprylates / pharmacology
  • Carrageenan / metabolism
  • Cell Movement / drug effects
  • Clofibrate / administration & dosage
  • Clofibrate / pharmacology
  • Complex Mixtures / administration & dosage*
  • Complex Mixtures / adverse effects
  • Complex Mixtures / chemistry
  • Cytokines / biosynthesis
  • Cytokines / genetics
  • Edema / chemically induced
  • Edema / drug therapy
  • Edema / immunology*
  • Fluorocarbons / administration & dosage
  • Fluorocarbons / pharmacology
  • Glucans / administration & dosage*
  • Glucans / adverse effects
  • Glucans / chemistry
  • Leukocytes / drug effects
  • Leukocytes / immunology
  • Leukocytes / metabolism
  • Leukocytes / pathology
  • Magnetic Resonance Spectroscopy
  • Male
  • Mice
  • Nitric Oxide / analysis
  • Peritonitis / chemically induced
  • Peritonitis / drug therapy
  • Peritonitis / immunology*
  • Pyrimidines / administration & dosage
  • Pyrimidines / pharmacology
  • Rats
  • Rats, Wistar
  • Thioglycolates / metabolism

Substances

  • Anti-Inflammatory Agents
  • Caprylates
  • Complex Mixtures
  • Cytokines
  • Fluorocarbons
  • Glucans
  • Pyrimidines
  • Thioglycolates
  • Nitric Oxide
  • pirinixic acid
  • Carrageenan
  • perfluorooctanoic acid
  • Clofibrate