Increase in the level of P-glycoprotein mRNA expression in multidrug-resistant K562 cell lines treated with sodium butyrate is not accompanied with erythroid differentiation

Jpn J Cancer Res. 1990 Dec;81(12):1214-7. doi: 10.1111/j.1349-7006.1990.tb02681.x.


We examined the effect of hemin, sodium butyrate and mitomycin C on levels of P-glycoprotein mRNA in human myelogenous K562 cells by northern blot analysis. After treatment with sodium butyrate a dose-dependent increase of P-glycoprotein mRNA expression was observed in the adriamycin-resistant K562 and vincristine-resistant K562 lines. With 10 mM sodium butyrate, the level of P-glycoprotein mRNA reached 20 times that of control adriamycin-resistant K562 and with 30 mM sodium butyrate, it exceeded 5 times that of control vincristine-resistant K562. In contrast, hemin and mitomycin C had almost no effect on P-glycoprotein mRNA. In this experiment, since expression of P-glycoprotein mRNA was not necessarily accompanied with induction of erythroid differentiation, the increased amount of P-glycoprotein mRNA is unlikely to be a result of differentiation.

Publication types

  • Comparative Study

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Blotting, Northern
  • Butyrates / pharmacology*
  • Butyric Acid
  • Cell Differentiation / drug effects
  • Cell Line / drug effects
  • Cell Line / metabolism
  • Doxorubicin / pharmacology
  • Drug Resistance / genetics
  • Gene Expression / drug effects
  • Hemin / pharmacology
  • Humans
  • Membrane Glycoproteins / genetics*
  • Mitomycin
  • Mitomycins / pharmacology
  • RNA, Messenger / analysis*
  • Vincristine / pharmacology


  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Butyrates
  • Membrane Glycoproteins
  • Mitomycins
  • RNA, Messenger
  • Butyric Acid
  • Mitomycin
  • Vincristine
  • Hemin
  • Doxorubicin