Loss of the plant DEAD-box protein ISE1 leads to defective mitochondria and increased cell-to-cell transport via plasmodesmata

Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):17229-34. doi: 10.1073/pnas.0909229106. Epub 2009 Sep 21.

Abstract

Plants have intercellular channels, plasmodesmata (PD), that span the cell wall to enable cell-to-cell transport of micro- and macromolecules. We identified an Arabidopsis thaliana embryo lethal mutant increased size exclusion limit 1 (ise1) that results in increased PD-mediated transport of fluorescent tracers. The ise1 mutants have a higher frequency of branched and twinned PD than wild-type embryos. Silencing of ISE1 in mature Nicotiana benthamiana leaves also leads to increased PD transport, as monitored by intercellular movement of a GFP fusion to the tobacco mosaic virus movement protein. ISE1 encodes a putative plant-specific DEAD-box RNA helicase that localizes specifically to mitochondria. The N-terminal 100 aa of ISE1 specify mitochondrial targeting. Mitochondrial metabolism is compromised severely in ise1 mutant embryos, because their mitochondrial proton gradient is disrupted and reactive oxygen species production is increased. Although mitochondria are essential for numerous cell-autonomous functions, the present studies demonstrate that mitochondrial function also regulates the critical cell non-cell-autonomous function of PD.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Biological Transport
  • DEAD-box RNA Helicases / classification
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism*
  • Flowers / genetics
  • Flowers / metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Microscopy, Fluorescence
  • Mitochondria / metabolism*
  • Molecular Sequence Data
  • Mutation
  • Phylogeny
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Proteins / classification
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plants, Genetically Modified
  • Plasmodesmata / metabolism*
  • Protons
  • Reactive Oxygen Species / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Seeds / genetics
  • Seeds / metabolism
  • Sequence Homology, Amino Acid
  • Tobacco / genetics
  • Tobacco / metabolism

Substances

  • Plant Proteins
  • Protons
  • Reactive Oxygen Species
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • DEAD-box RNA Helicases