Systemic lupus erythematosus (SLE) is characterized by the presence of antinuclear antibodies (ANA). ANAs are aimed against native DNA, as well as histone proteins and small nuclear ribonucleoproteins, also could be targeted against the entire chromatin, consisted of nucleosomes. Herein, we assessed the frequency and the concentration of anti-nucleosome antibodies (aNS) in a group of 107 Hungarian patients with SLE. Moreover, correlations between these autoantibody concentrations and disease activity, as well as organ manifestations, mainly renal symptoms were evaluated. The occurrence of positive aNS, anti-dsDNA and anti-histone (aH) antibodies were 39.2, 28.0 and 47.6%, respectively. All the three autoantibodies were represented with significantly higher titers in patients with lupus nephritis. A positive correlation was found between the concentration of these autoantibodies and disease activity. The long disease duration (mean 8.5 years) and consequent low disease activity (mean disease activity index: 3.28) could be the reason for the relatively low occurrence of ANAs amongst patients. Besides anti-dsDNA, the assessment of aNS and aH antibodies could be useful in the diagnosis and follow-up of SLE. The authors discuss the possible role of aNS autoantibodies in the pathogenesis of lupus nephritis.