Evaluation of antidiabetic activity of polysaccharide isolated from Phellinus linteus in non-obese diabetic mouse

Int Immunopharmacol. 2010 Jan;10(1):72-8. doi: 10.1016/j.intimp.2009.09.024. Epub 2009 Oct 5.


Polysaccharide (PLP) isolated from Phellinus linteus inhibits tumor growth and metastasis by enhancing immune functions of macrophages, dendritic cells, NK cells, T cells, and B cells. Here, we report that PLP can inhibit the development of autoimmune diabetes in non-obese diabetic (NOD) mice. Although 80% of the NOD mice had developed diabetes by 24 weeks of age, none of the PLP-treated NOD mice developed diabetes. The mean blood glucose levels were 110mg/dl in PLP-treated mice and 499mg/dl in control NOD mice. Histological examination of the pancreatic islets revealed that most of the islets isolated from PLP-treated mice were less infiltrated with lymphocytes compared with those of control mice. Spleen cells from diabetic NOD mice could adaptively transfer diabetes into NOD/SCID mice, but those from PLP-treated NOD mice showed delayed transfer of diabetes. PLP inhibited the expression of inflammatory cytokines, including IFN-gamma, IL-2, and TNF-alpha by Th1 cells and macrophages, but up-regulated IL-4 expression by Th2 cells in NOD mice. PLP did not prevent streptozotocin-induced diabetic development in ICR mice. Taken together, these results suggest that PLP inhibits the development of autoimmune diabetes by regulating cytokine expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Blood Glucose / drug effects*
  • Blood Glucose / immunology
  • Body Weight / drug effects
  • Cytokines / genetics
  • Cytokines / immunology
  • Cytokines / metabolism*
  • Diabetes Mellitus, Type 1 / chemically induced
  • Diabetes Mellitus, Type 1 / drug therapy*
  • Diabetes Mellitus, Type 1 / physiopathology
  • Disease Progression
  • Female
  • Fungi
  • Humans
  • Mice
  • Mice, Inbred ICR
  • Mice, Inbred NOD
  • Pancreas / drug effects*
  • Pancreas / immunology
  • Pancreas / metabolism
  • Pancreas / pathology
  • Phellinus
  • Plant Extracts
  • Polysaccharides / administration & dosage*
  • Polysaccharides / adverse effects
  • Streptozocin / administration & dosage
  • Th1 Cells / drug effects
  • Th1 Cells / immunology
  • Th1 Cells / transplantation
  • Th2 Cells / drug effects
  • Th2 Cells / immunology
  • Th2 Cells / transplantation


  • Blood Glucose
  • Cytokines
  • Phellinus linteus extract
  • Plant Extracts
  • Polysaccharides
  • Streptozocin