Bacterial chemoattraction towards jasmonate plays a role in the entry of Dickeya dadantii through wounded tissues

Mol Microbiol. 2009 Nov;74(3):662-71. doi: 10.1111/j.1365-2958.2009.06888.x. Epub 2009 Oct 8.

Abstract

Jasmonate is a key signalling compound in plant defence that is synthesized in wounded tissues. In this work, we have found that this molecule is also a strong chemoattractant for the phythopathogenic bacteria Dickeya dadantii (ex-Erwinia chysanthemi). Jasmonic acid induced the expression of a subset of bacterial genes possibly involved in virulence/survival in the plant apoplast and bacterial cells pre-treated with jasmonate showed increased virulence in chicory and Saintpaulia leaves. We also showed that tissue wounding induced bacterial spread through the leaf surface. Moreover, the jasmonate-deficient aos1 Arabidopsis thaliana mutant was more resistant to bacterial invasion by D. dadantii than wild-type plants. These results are consistent with the hypothesis that sensing jasmonic acid by this bacterium helps the pathogen to ingress inside plant tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Carbon-Sulfur Lyases / genetics
  • Carbon-Sulfur Lyases / metabolism
  • Chemotaxis / drug effects*
  • Chicory / genetics
  • Chicory / metabolism
  • Chicory / microbiology
  • Cyclopentanes / pharmacology*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae / pathogenicity
  • Enterobacteriaceae / physiology*
  • Gene Expression Regulation, Bacterial
  • Gene Regulatory Networks
  • Genes, Bacterial
  • Genome, Bacterial
  • Iron-Sulfur Proteins / genetics
  • Iron-Sulfur Proteins / metabolism
  • Magnoliopsida / genetics
  • Magnoliopsida / metabolism
  • Mutation
  • Oxylipins / pharmacology*
  • Plant Diseases / genetics
  • Plant Diseases / microbiology
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Leaves / microbiology
  • Plants / genetics
  • Plants / metabolism
  • RNA, Bacterial / genetics
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Signal Transduction / genetics
  • Virulence / genetics
  • Virulence Factors / genetics

Substances

  • Bacterial Proteins
  • Cyclopentanes
  • Iron-Sulfur Proteins
  • Oxylipins
  • RNA, Bacterial
  • Repressor Proteins
  • Virulence Factors
  • jasmonic acid
  • Carbon-Sulfur Lyases