Development and evaluation of a multiplex polymerase chain reaction assay to identify Salmonella serogroups and serotypes

Nora Cardona-Castro; Miryan Sánchez-Jiménez; Lelia Lavalett; Nélida Múñoz; Jaime Moreno

doi:10.1016/j.diagmicrobio.2009.07.003

Development and evaluation of a multiplex polymerase chain reaction assay to identify Salmonella serogroups and serotypes

Diagn Microbiol Infect Dis. 2009 Nov;65(3):327-30. doi: 10.1016/j.diagmicrobio.2009.07.003.

Authors

Nora Cardona-Castro¹, Miryan Sánchez-Jiménez, Lelia Lavalett, Nélida Múñoz, Jaime Moreno

Affiliation

¹ Laboratorio de Microbiología, Instituto Colombiano de Medicina Tropical - Universidad CES Cra 43 A #52 Sur 99, Medellín, Colombia. ncardona@ces.edu.co

PMID: 19822272
DOI: 10.1016/j.diagmicrobio.2009.07.003

Abstract

To improve limitations of Salmonella serotyping, 2 multiplex polymerase chain reaction (M-PCR) were developed using a strategy that identifies first the genes encoding serogroups (rfbJ, wzx). According to the serogroup determined, a second M-PCR identifies serotype (fliC, fljB, wcdB, and sdf-I sequence). Standardization and evaluation of both M-PCRs were carried out.

MeSH terms

Bacterial Proteins / genetics
DNA Primers
Polymerase Chain Reaction / methods*
Reproducibility of Results
Salmonella / classification*
Salmonella / genetics
Serotyping / methods*

Substances

Bacterial Proteins
DNA Primers