A small genomic region containing several loci required for gastrulation in Drosophila

PLoS One. 2009 Oct 13;4(10):e7437. doi: 10.1371/journal.pone.0007437.


Genetic screens in Drosophila designed to search for loci involved in gastrulation have identified four regions of the genome that are required zygotically for the formation of the ventral furrow. For three of these, the genes responsible for the mutant phenotypes have been found. We now describe a genetic characterization of the fourth region, which encompasses the cytogenetic interval 24C3-25B, and the mapping of genes involved in gastrulation in this region. We have determined the precise breakpoints of several existing deficiencies and have generated new deficiencies. Our results show that the region contains at least three different loci associated with gastrulation effects. One maternal effect gene involved in ventral furrow formation maps at 24F but could not be identified. For a second maternal effect gene which is required for germ band extension, we identify a candidate gene, CG31660, which encodes a G protein coupled receptor. Finally, one gene acts zygotically in ventral furrow formation and we identify it as Traf4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Cytogenetics
  • Drosophila Proteins / genetics
  • Drosophila melanogaster / embryology*
  • Drosophila melanogaster / genetics*
  • Female
  • Gastrulation*
  • Gene Deletion
  • Genes, Insect
  • Genetic Techniques
  • Genomics
  • Heterozygote
  • Male
  • Models, Genetic
  • Phenotype


  • Drosophila Proteins