Abstract
A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amides / chemistry*
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Amino Acids / metabolism
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Anti-HIV Agents / metabolism
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Bacteriophage T7 / metabolism*
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Cytarabine / pharmacology
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Drug Delivery Systems
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Drug Design
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Humans
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Peptide Library
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Phosphoric Acids / chemistry*
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Virus Replication
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Zidovudine / metabolism*
Substances
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Amides
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Amino Acids
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Anti-HIV Agents
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Peptide Library
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Phosphoric Acids
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Cytarabine
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Zidovudine
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phosphoramidic acid