Hydrolytic activity of histidine-containing octapeptides in water identified by quantitative screening of a combinatorial library

Org Biomol Chem. 2009 Nov 7;7(21):4362-8. doi: 10.1039/b904415a. Epub 2009 Aug 19.


A quantitative on-bead screening of a combinatorial octapeptide library revealed catalysts with hydrolytic activity in water. Histidine is an essential amino acid but the catalytic activity as well as the substrate binding affinity is also dependent on the sequence of the octapeptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Buffers
  • Combinatorial Chemistry Techniques
  • Esterases / metabolism
  • Histidine*
  • Hydrolysis
  • Oligopeptides / analysis
  • Oligopeptides / chemistry*
  • Oligopeptides / metabolism*
  • Water*


  • Buffers
  • Oligopeptides
  • Water
  • Histidine
  • Esterases