Ly6d Marks the Earliest Stage of B-cell Specification and Identifies the Branchpoint Between B-cell and T-cell Development

Genes Dev. 2009 Oct 15;23(20):2376-81. doi: 10.1101/gad.1836009.

Abstract

Common lymphoid progenitors (CLPs) clonally produce both B- and T-cell lineages, but have little myeloid potential in vivo. However, some studies claim that the upstream lymphoid-primed multipotent progenitor (LMPP) is the thymic seeding population, and suggest that CLPs are primarily B-cell-restricted. To identify surface proteins that distinguish functional CLPs from B-cell progenitors, we used a new computational method of Mining Developmentally Regulated Genes (MiDReG). We identified Ly6d, which divides CLPs into two distinct populations: one that retains full in vivo lymphoid potential and produces more thymocytes at early timepoints than LMPP, and another that behaves essentially as a B-cell progenitor.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Ly / genetics
  • Antigens, Ly / metabolism*
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / metabolism*
  • Cell Differentiation*
  • Cells, Cultured
  • GPI-Linked Proteins
  • Gene Expression Regulation, Developmental
  • Lymphoid Progenitor Cells / cytology*
  • Mice
  • Mice, Inbred C57BL
  • T-Lymphocytes / cytology*
  • T-Lymphocytes / metabolism*

Substances

  • Antigens, Ly
  • GPI-Linked Proteins
  • Ly6d protein, mouse