Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 113 (2), 114-20

Dot Immunogold Filtration Assay (DIGFA) With Multiple Native Antigens for Rapid Serodiagnosis of Human Cystic and Alveolar Echinococcosis

Affiliations

Dot Immunogold Filtration Assay (DIGFA) With Multiple Native Antigens for Rapid Serodiagnosis of Human Cystic and Alveolar Echinococcosis

Xiaohui Feng et al. Acta Trop.

Abstract

A new 3-min rapid dot immunogold filtration assay (DIGFA) for serodiagnosis of human cystic and alveolar echinococcosis was developed using four native antigen preparations: crude and partially purified hydatid cyst fluid extracts from Echinococcus granulosus (EgCF and AgB), E. granulosus protoscolex extract (EgP) and Echinococcus multilocularis metacestode antigen (Em2). The overall sensitivity of DIGFA in a hospital diagnostic setting was 80.7% for human cystic echinococcosis (CE) (n=857) and 92.9% for human alveolar echinococcosis (AE) (n=42). Highest specificity was 93.4% with AgB extract for CE, and 90.3% with Em2 antigen for AE when CE versus AE cross-reactivity was excluded. Anti-AgB antibodies were present in 35.5% of AE cases and anti-Em2 in 7.4% of CE cases. In endemic communities in northwest China screened for echinococcosis, the sensitivity of DIGFA ranged from 71.8% to 90.7% in comparison to abdominal ultrasound; specificity for CE using AgB was 94.6% and for AE using Em2 was 97.1%. This simple eye-read rapid test can be used for both clinical diagnostic support, as well as in conjunction with ultrasound for mass screening in endemic CE and AE areas.

Similar articles

See all similar articles

Cited by 20 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback