Antioxidant and anti-inflammatory properties of cancer preventive peptide lunasin in RAW 264.7 macrophages

Biochem Biophys Res Commun. 2009 Dec 18;390(3):803-8. doi: 10.1016/j.bbrc.2009.10.053. Epub 2009 Oct 15.

Abstract

Oxidative stress and inflammation are two of the most critical factors implicated in carcinogenesis and other degenerative disorders. We have investigated how lunasin, a known anti-cancer seed peptide, affect these factors. This peptide inhibits linoleic acid oxidation and acts as 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenger. Furthermore, using LPS-stimulated RAW 264.7 macrophages, we have demonstrated that lunasin reduces, in a significant dose-dependent manner, the production of reactive oxygen species (ROS) by LPS-induced macrophages. Lunasin also inhibits the release of pro-inflammatory cytokines (tumor necrosis factor-alpha [TNF-alpha] and interleukine-6 [IL-6]). On the basis of these potent antioxidant and anti-inflammatory properties, we propose lunasin not only as a cancer preventive and therapeutic agent but also as an agent against other inflammatory-related disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Anticarcinogenic Agents / pharmacology
  • Antioxidants / pharmacology*
  • Cell Line
  • Linoleic Acid / metabolism
  • Macrophages / drug effects*
  • Mice
  • Oxidation-Reduction / drug effects
  • Reactive Oxygen Species / metabolism
  • Soybean Proteins / metabolism
  • Soybean Proteins / pharmacology*

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Anticarcinogenic Agents
  • Antioxidants
  • GM2S-1 protein, Glycine max
  • Reactive Oxygen Species
  • Soybean Proteins
  • Linoleic Acid