Membrane protein assembly into Nanodiscs

FEBS Lett. 2010 May 3;584(9):1721-7. doi: 10.1016/j.febslet.2009.10.024. Epub 2009 Oct 16.


Nanodiscs are soluble nanoscale phospholipid bilayers which can self-assemble integral membrane proteins for biophysical, enzymatic or structural investigations. This means for rendering membrane proteins soluble at the single molecule level offers advantages over liposomes or detergent micelles in terms of size, stability, ability to add genetically modifiable features to the Nanodisc structure and ready access to both sides of the phospholipid bilayer domain. Thus the Nanodisc system provides a novel platform for understanding membrane protein function. We provide an overview of the Nanodisc approach and document through several examples many of the applications to the study of the structure and function of integral membrane proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Humans
  • Lipid Bilayers / chemistry
  • Lipid Bilayers / metabolism*
  • Membrane Lipids / chemistry
  • Membrane Lipids / metabolism
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism*
  • Membrane Proteins / physiology
  • Models, Biological
  • Models, Molecular
  • Nanostructures* / chemistry
  • Protein Multimerization / physiology*
  • Surface Properties


  • Lipid Bilayers
  • Membrane Lipids
  • Membrane Proteins