Intact and injured endothelial cells differentially modulate postnatal murine forebrain neural stem cells

Neurobiol Dis. 2010 Jan;37(1):218-27. doi: 10.1016/j.nbd.2009.10.008. Epub 2009 Oct 23.


Neural stem cells (NSCs) persist in the forebrain subventricular zone (SVZ) within a niche containing endothelial cells. Evidence suggests that endothelial cells stimulate NSC expansion and neurogenesis. Experimental stroke increases neurogenesis and angiogenesis, but how endothelial cells influence stroke-induced neurogenesis is unknown. We hypothesized intact or oxygen-glucose deprived (OGD) endothelial cells secrete factors that enhance neurogenesis. We co-cultured mouse SVZ neurospheres (NS) with endothelial cells, or differentiated NS in endothelial cell-conditioned medium (ECCM). NS also were expanded in ECCM from OGD-exposed (OGD-ECCM) endothelial cells to assess injury effects. ECCM significantly increased NS production. NS co-cultured with endothelial cells or ECCM generated more immature-appearing neurons and oligodendrocytes, and astrocytes with radial glial-like/reactive morphology than controls. OGD-ECCM stimulated neuroblast migration and yielded neurons with longer processes and more branching. These data indicate that intact and injured endothelial cells exert differing effects on NSCs, and suggest targets for stimulating regeneration after brain insults.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / physiology
  • Cell Hypoxia / physiology
  • Cell Line
  • Cell Movement / physiology
  • Cells, Cultured
  • Coculture Techniques
  • Culture Media, Conditioned
  • Endothelial Cells / metabolism
  • Endothelial Cells / physiology*
  • Fluorescent Antibody Technique
  • Glucose / deficiency
  • Mice
  • Mice, Inbred Strains
  • Neurogenesis / physiology
  • Neurons / cytology
  • Neurons / physiology*
  • Oligodendroglia / physiology
  • Stem Cell Niche / growth & development*
  • Stem Cell Niche / physiology*
  • Stem Cells / physiology*


  • Culture Media, Conditioned
  • Glucose