Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease

Gastroenterology. 1991 Jan;100(1):3-12. doi: 10.1016/0016-5085(91)90575-6.


Expression of class II antigens on human intestinal epithelial cells was assessed using a sensitive avidinbiotin-peroxidase technique. HLA-DR was present predominantly in the normal small bowel with diminished but evident expression in the colon. HLA-DP staining was less prominent, and HLA-DQ was absent. In inflammatory bowel disease the expression of both HLA-DR and HLA-DP was increased, but that for HLA-DQ remained absent, suggesting an inherent defect in the ability of intestinal epithelial cells to express HLA-DQ. In related experiments, an interferon gamma-treated malignant epithelial cell line T84 also failed to stain for HLA-DQ and HLA-DP despite the presence of HLA-DR. Isolated RNAs for all three subclasses of HLA-D were detectable by slot-blot analysis, suggesting that the lack of HLA-DQ expression relates to posttranscriptional defects in intestinal epithelium. These and other differences with conventional class II antigen-positive accessory cells (macrophages/B cells) may help to explain the unique properties of intestinal epithelial cells as antigen-presenting cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / immunology
  • Colonic Neoplasms / immunology
  • Gene Expression Regulation
  • HLA-D Antigens / biosynthesis*
  • HLA-D Antigens / genetics
  • Humans
  • Inflammatory Bowel Diseases / immunology*
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / immunology*
  • Tumor Cells, Cultured


  • HLA-D Antigens