Brassinosteroids protect Lycopersicon esculentum from cadmium toxicity applied as shotgun approach

Protoplasma. 2010 Mar;239(1-4):3-14. doi: 10.1007/s00709-009-0075-2. Epub 2009 Oct 17.

Abstract

Surface-sterilized seeds of two tomato cultivars (cv. K-25 and Sarvodya) were soaked in 100 microM CdCl(2) for 8 h (shotgun approach). The resulting 59-day-old seedlings were sprayed with 10(-8) M of 28-homobrassinolide (HBL) or 24-epibrassinolide (EBL) to their foliage. Both cultivars showed significantly different response to Cd stress. Cadmium severely restricted the growth, photosynthetic efficiency, and activity of nitrate reductase (E.C. 1.6.6.1) and carbonic anhydrase (E.C. 4.2.1.1) in Sarvodya as compared to K-25. However, the activities of antioxidative enzymes were significantly higher in K-25. This result may be considered an indication of better tolerance of the K-25 cultivars to Cd stress. Moreover, the spray of both the brassinosteroids (HBL/EBL) were found very effective in neutralizing the adverse effects generated by metals that reflect in better photosynthetic performance by the cultivars. An interesting aspect of this study is that HBL or EBL spray caused a further increase in proline content and antioxidative enzyme activities, which were already enhanced by Cd stress. This effect of brassinosteroids (HBL/EBL) was more pronounced in K-25 than in Sarvodya, representing the tolerance and adoptable behavior of K-25.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / metabolism
  • Ascorbic Acid / metabolism
  • Brassinosteroids
  • Cadmium / toxicity*
  • Carotenoids / metabolism
  • Cholestanols / pharmacology*
  • Cholestanones / pharmacology*
  • Lycopene
  • Lycopersicon esculentum / anatomy & histology
  • Lycopersicon esculentum / drug effects*
  • Lycopersicon esculentum / physiology
  • Nitrate Reductase / metabolism
  • Photosynthesis / drug effects
  • Photosynthesis / physiology
  • Plant Growth Regulators / pharmacology*
  • Plant Proteins / metabolism
  • Proline / metabolism
  • Seedlings / drug effects*
  • Seeds / drug effects
  • Seeds / metabolism
  • Soil Pollutants / toxicity
  • Steroids, Heterocyclic / pharmacology*
  • Vitamins / metabolism
  • beta Carotene / metabolism

Substances

  • Antioxidants
  • Brassinosteroids
  • Cholestanols
  • Cholestanones
  • Plant Growth Regulators
  • Plant Proteins
  • Soil Pollutants
  • Steroids, Heterocyclic
  • Vitamins
  • Cadmium
  • beta Carotene
  • Carotenoids
  • homobrassinolide
  • Proline
  • Nitrate Reductase
  • Ascorbic Acid
  • Lycopene
  • brassinolide