REBASE--a database for DNA restriction and modification: enzymes, genes and genomes

Nucleic Acids Res. 2010 Jan;38(Database issue):D234-6. doi: 10.1093/nar/gkp874. Epub 2009 Oct 21.


REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction-modification (R-M). It contains fully referenced information about recognition and cleavage sites, isoschizomers, neoschizomers, commercial availability, methylation sensitivity, crystal and sequence data. Experimentally characterized homing endonucleases are also included. The fastest growing segment of REBASE contains the putative R-M systems found in the sequence databases. Comprehensive descriptions of the R-M content of all fully sequenced genomes are available including summary schematics. The contents of REBASE may be browsed from the web ( and selected compilations can be downloaded by ftp ( Additionally, monthly updates can be requested via email.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Computational Biology / methods*
  • Computational Biology / trends
  • DNA Modification Methylases / genetics*
  • DNA Restriction Enzymes / genetics*
  • Databases, Genetic*
  • Databases, Protein
  • Genome, Archaeal
  • Genome, Bacterial
  • Genomics
  • Information Storage and Retrieval / methods
  • Internet
  • Protein Structure, Tertiary
  • Software
  • Substrate Specificity


  • DNA Modification Methylases
  • DNA Restriction Enzymes