Incubation alone is adequate as a culturing technique for cardiac rhythm management devices

J Clin Microbiol. 2009 Dec;47(12):4168-70. doi: 10.1128/JCM.01354-09. Epub 2009 Oct 21.


There exist no standardized methods for culturing cardiac rhythm management devices. To identify the most optimal culturing method, we compared various techniques that comprise vortex, sonication, and incubation or combinations thereof. Incubation alone yielded bacterial colony counts similar to those of other culturing combinations and is the least labor-intensive.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques / methods*
  • Colony Count, Microbial
  • Culture Media
  • Defibrillators, Implantable / microbiology*
  • Equipment Contamination*
  • Humans
  • Methicillin-Resistant Staphylococcus aureus / growth & development
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification
  • Pacemaker, Artificial / microbiology*
  • Pseudomonas aeruginosa / growth & development
  • Pseudomonas aeruginosa / isolation & purification
  • Sonication


  • Culture Media