Inhibition of HSP90 with pochoximes: SAR and structure-based insights

Chembiochem. 2009 Nov 23;10(17):2753-9. doi: 10.1002/cbic.200900494.


The pochoximes, based on the radicicol pharmacophore, are potent inhibitors of heat shock protein 90 (HSP90) that retain their activity in vivo. Herein we report an extended library that broadly explores the structure-activity relationship (SAR) of the pochoximes with four points of diversity. Several modifications were identified that afford improved cellular efficacy, new opportunities for conjugation, and further diversifications. Cocrystal structures of pochoximes A and B with HSP90 show that pochoximes bind to a different conformation of HSP90 than radicicol and provide a rationale for the enhanced affinity of the pochoximes relative to radicicol and the pochonins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / metabolism
  • HSP90 Heat-Shock Proteins / antagonists & inhibitors*
  • HSP90 Heat-Shock Proteins / chemistry
  • HSP90 Heat-Shock Proteins / metabolism
  • Humans
  • Macrolides / chemistry
  • Macrolides / metabolism
  • Molecular Structure
  • Oximes / chemical synthesis
  • Oximes / chemistry*
  • Protein Structure, Tertiary
  • Small Molecule Libraries
  • Structure-Activity Relationship


  • Enzyme Inhibitors
  • HSP90 Heat-Shock Proteins
  • Macrolides
  • Oximes
  • Small Molecule Libraries
  • monorden