Co-appearance of autoantibody-producing B220(low) B cells with NKT cells in the course of hepatic injury

Cell Immunol. 2010;260(2):105-12. doi: 10.1016/j.cellimm.2009.09.009.


Severe hepatic injury is induced by Concanavalin A (Con A) administration in mice, the major effector cells being CD4(+) T cells, NKT cells and macrophages. Since autologous lymphocyte subsets are associated with tissue damage, Con A-induced hepatic injury is considered to be autoimmune hepatitis. However, it has remained to be investigated how autoantibodies and B-1 cells are responsible for this phenomenon. In this study, it was demonstrated that autoantibodies which were detected using Hep-2 cells in immunofluorescence tests and using double-strand (ds) DNA in the ELISA method, appeared after Con A administration (a peak at day 14). Moreover, autoantibody-producing B220(low) cells (i.e., B-1 cells) also appeared at this time. Purified B220(low) cells were found to have a potential to produce autoantibodies. These results suggest that Con A-induced hepatic injury indeed includes the mechanism of autoimmune hepatitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood
  • Animals
  • Autoantibodies / blood
  • Autoantibodies / immunology*
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Cell Line, Tumor
  • Concanavalin A
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Granulocytes / cytology
  • Granulocytes / immunology
  • Hepatitis, Animal / blood
  • Hepatitis, Animal / chemically induced
  • Hepatitis, Animal / immunology*
  • Humans
  • Immunohistochemistry
  • Lymphocyte Count
  • Macrophages / cytology
  • Macrophages / immunology
  • Mice
  • Mice, Inbred C57BL
  • Natural Killer T-Cells / cytology
  • Natural Killer T-Cells / immunology*
  • Spleen / cytology
  • Spleen / immunology
  • Time Factors


  • Autoantibodies
  • Concanavalin A
  • Alanine Transaminase