Investigation of the role of tumour necrosis factor-{alpha}, interleukin-1{beta}, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis

Lab Anim. 2010 Apr;44(2):143-9. doi: 10.1258/la.2009.0080132. Epub 2009 Oct 26.

Abstract

Collagen-induced arthritis (CIA) in rats is a widely used preclinical animal model of rheumatoid arthritis (RA). However, CIA development in Sprague-Dawley (SD) rats is less severe in terms of inflammatory response compared with other strains. Therefore, a modified CIA model called MCIA, using N-acetylmuramyl dipeptide (MDP), has been developed in the less sensitive SD rat strains. This work was conducted to better understand the immunopathological role and contributions of the pro-inflammatory T-helper type 1 (Th-1) cytokines and inflammatory mediators, interleukin-1 (IL-1beta), tumour necrosis factor-alpha (TNF-alpha) and nitric oxide (NO); the anti-inflammatory T-helper type 2 (Th-2) cytokine, IL-10 and autoantibodies such as rheumatoid factor (RF)-immunoglobulin M (IgM) in this newly developed RA model. TNF-alpha, NO and RF-IgM levels were significantly increased, while IL-1beta levels were not affected in this MCIA rat model. The levels of IL-10 were lower than the baseline when compared with controls.

In conclusion: (1) the immunological features represented in the MCIA rat model favour the Th-1 cytokine profile over Th-2 and (2) RF-IgM can be used as a diagnostic test in preclinical RA models.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthritis, Rheumatoid / immunology*
  • Disease Models, Animal*
  • Immunoglobulin M / immunology
  • Immunoglobulin M / metabolism*
  • Interleukin-10 / immunology
  • Interleukin-10 / metabolism*
  • Interleukin-1beta / immunology
  • Interleukin-1beta / metabolism*
  • Male
  • Nitric Oxide / immunology
  • Nitric Oxide / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Rheumatoid Factor / immunology
  • Rheumatoid Factor / metabolism*
  • Th1 Cells / immunology
  • Th1 Cells / metabolism
  • Tumor Necrosis Factor-alpha / immunology
  • Tumor Necrosis Factor-alpha / metabolism*

Substances

  • Immunoglobulin M
  • Interleukin-1beta
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Nitric Oxide
  • Rheumatoid Factor