Abstract
Using competition mobility shift, methylation interference, and proteolytic clipping DNA binding assays, we demonstrate that the protein binding the major histocompatibility complex A beta CCAAT box is indistinguishable from the protein previously named NF-Y, which binds the major histocompatibility complex E alpha CCAAT box. Although the two CCAAT boxes share the same 10-base core sequence, termed the Y box, their flanking sequences, known to be important for binding, are very different.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins / metabolism*
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Histocompatibility Antigens Class II / genetics*
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In Vitro Techniques
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Major Histocompatibility Complex*
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Mice
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Molecular Sequence Data
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Oligonucleotides / chemistry
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Promoter Regions, Genetic*
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Regulatory Sequences, Nucleic Acid*
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Transcription Factors / metabolism*
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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Histocompatibility Antigens Class II
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Oligonucleotides
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Transcription Factors