PDGF A-chain gene is expressed by mammalian neurons during development and in maturity

Cell. 1991 Jan 11;64(1):209-16. doi: 10.1016/0092-8674(91)90222-k.


Platelet-derived growth factor (PDGF) may be a critical factor in the temporal differentiation of glial elements in the mammalian central nervous system. We have used in situ hybridization and immunoperoxidase staining to investigate the localization of PDGF A and have observed high levels of PDGF A-chain mRNA and immunoreactive PDGF A in neurons of embryonic and adult mice. PDGF A-chain expression was shown to be developmentally regulated and tissue specific. Every neuronal population examined in the central and peripheral nervous systems expresses PDGF A transcripts. Variable, significantly weaker signals are observed in glial cells. In contrast to known neurotrophic factors, the PDGF A transcripts are widely distributed among neurons. This generalized distribution of PDGF A transcripts, together with the known effects of PDGF on glial cells in vitro, suggests a unique role of neurons in regulating the proliferation and differentiation of glial cells in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Brain / cytology
  • Brain / embryology*
  • Brain / metabolism
  • Gene Expression Regulation*
  • Genes*
  • Gestational Age
  • Immunoenzyme Techniques
  • Macromolecular Substances
  • Mice
  • Mice, Inbred BALB C
  • Neurons / cytology
  • Neurons / metabolism*
  • Nucleic Acid Hybridization
  • Platelet-Derived Growth Factor / genetics*
  • RNA Probes
  • RNA, Messenger / analysis*
  • RNA, Messenger / genetics
  • Spinal Cord / cytology
  • Spinal Cord / embryology*
  • Spinal Cord / metabolism
  • Sulfur Radioisotopes


  • Macromolecular Substances
  • Platelet-Derived Growth Factor
  • RNA Probes
  • RNA, Messenger
  • Sulfur Radioisotopes