IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : I. ESTIMATION OF A AND B ISOANTIBODIES IN HUMAN SERUM BY THE QUANTITATIVE PRECIPITIN METHOD

E A Kabat; A E Bezer

doi:10.1084/jem.82.3.207

IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : I. ESTIMATION OF A AND B ISOANTIBODIES IN HUMAN SERUM BY THE QUANTITATIVE PRECIPITIN METHOD

J Exp Med. 1945 Sep 1;82(3):207-15. doi: 10.1084/jem.82.3.207.

Authors

E A Kabat¹, A E Bezer

Affiliation

¹ Department of Neurology, College of Physicians and Surgeons, Columbia University and the Neurological Institute of New York.

Abstract

1. With purified blood group A and B specific substances from the gastric mucin of pigs precipitin reactions can be obtained in sera containing homologous isoagglutinins. By microquantitative precipitin technics the anti-A and anti-B content of normal and immune isoagglutinin-containing sera can be estimated on a weight basis. 2. There was a general correlation between antibody N precipitable from the sera of groups O and B by A substance and the agglutinin titer for A erythrocytes before and after immunization with blood group substances. 3. The reaction between A substance and its homologous isoagglutinin present in the sera of immunized individuals was of the same type as in other antigen-antibody systems.